Author : Jing Li, Juan Wang, Jinxin Li, Dahui Liu, Hongfa Li, Wenyuan Gao, Jianli Li, Shujie Liu.

Address : (Tianjin Key Laboratory for Modern Drug Delivery and High Efficiency, School of Pharmaceutical Science and Technology, Tianjin University. State Key Laboratory Breeding Base of Dao-di Herbs, China Academy of Chinese Medical Sciences, Institute of Medicinal Plants, Yunnan Academy of Agricultural Sciences, Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, Tianjin University of Science and Technology).

Abstract : Aspergillus niger Enhance Bioactive Compounds Biosynthesis As Well As Expression of Functional Genes in Adventitious Roots of Glycyrrhiza uralensis Fisch.

Journal : Applied Biochemistry and Biotechnology,

Volume No. : Volume 178(3)

Publish Year : 2016

Page No. : 576-593

Main Data : In the present study, the culture conditions for the accumulation of Glycyrrhiza uralensis adventitious root metabolites in balloon-type bubble bioreactors (BTBBs) have been optimized. The results of the culture showed that the best culture conditions were a cone angle of 90° bioreactor and 0.4–0.6–0.4-vvm aeration volume. Aspergillus niger can be used as a fungal elicitor to enhance the production of defense compounds in plants. With the addition of a fungal elicitor (derived from Aspergillus niger), the maximum accumulation of total flavonoids (16.12 mg g^-1) and glycyrrhetinic acid (0.18 mg g^-1) occurred at a dose of 400 mg L^-1 of Aspergillus niger resulting in a 3.47-fold and 1.8-fold increase over control roots. However, the highest concentration of polysaccharide (106.06 mg g^-1) was achieved with a mixture of elicitors (Aspergillus niger and salicylic acid) added to the medium, resulting in a 1.09-fold increase over Aspergillus niger treatment alone. Electrospray ionization tandem mass spectrometry (ESI-MSⁿ) analysis was performed, showing that seven compounds were present after treatment with the elicitors, including uralsaponin B, licorice saponin B₂, liquiritin, and (3R)-vestitol, only identified in the mixed elicitor treatment group. It has also been found that elicitors (Aspergillus niger and salicylic acid) significantly upregulated the expression of the cinnamate 4-hydroxylase (C4H), ß-amyrin synthase (ß-AS), squalene epoxidase (SE) and a cytochrome P450 monooxygenase (CYP72A154) genes, which are involved in the biosynthesis of bioactive compounds, and increased superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD) activity. Keywords: Glycyrrhiza uralensis Fisch; Adventitious root; Aspergillus niger; HPLC-ESI-MSⁿ; Anti-oxidant enzymes; Gene